Detection of amylase and urease in mesophilic bacteria isolated from sludge of sewage treatment Mangueira, Recife-Pernambuco

Abstract

Sewage sludge is a residue generated during the process of biological treatment of wastewater, being rich in organic matter and essential nutrients for plants and microorganisms. Bacteria are abundant in soil microbes responsible for various enzymatic transformations, assisting in the processes of decomposition and mineralization of various nutrients. Were collected sewage sludge treatment plant at Mangueira, Recife-PE for the isolation, identification and detection enzyme (amylase and urease) of mesophilic bacteria at temperatures of 28 and 37 oC. The results showed a temperature of 28oC, was presented the highest number of isolates. At both temperatures, were found pathogenic bacteria (Escherichia coli, Shigella sp, Pseudomonas sp, Alcaligenes sp, Enterobacter sp.) In enzymatic assays, the best results were obtained at 28 ° C, where the Streptococcus sp (CPU 0120) obtained halo of 70 mm for amylase. The arrest of urease was observed at both temperatures.