Comparação entre duas técnicas de análise de fibras elásticas pulmonares em coelhos prematuros

Fernanda Polastri Thereza; Mariana Mendes Silva; Rodrigo Leonel dos Santos; Bruno Garcia Aquino; Celso Moura Rebello; Renata Suman Mascaretti; Roberta Munhoz Manzano

doi:10.5585/conssaude.v11n2.3278

Authors

Fernanda Polastri Thereza Faculdades Integradas de Bauru
Mariana Mendes Silva Faculdades Integradas de Bauru
Rodrigo Leonel dos Santos Faculdades Integradas de Bauru
Bruno Garcia Aquino Faculdades Integradas de Bauru
Celso Moura Rebello Universidade de São Paulo
Renata Suman Mascaretti Universidade de São Paulo
Roberta Munhoz Manzano Universidade de São Paulo / Faculdades Integradas de Bauru

DOI:

https://doi.org/10.5585/conssaude.v11n2.3278

Keywords:

Bronchopulmonary dysplasia, Elastic tissue, Infant, premature.

Abstract

Introduction: Bronchopulmonary dysplasia is characterized by a blockage in lung development. Objective: To compare the efficiency of two techniques of analysis of elastic fibers in lungs of premature rabbits. Methods: Preterm rabbits born on the 28th and 29th days of gestation were subjected to ambient air or oxygen for 11 days, randomized into four groups, Ar 28 (n=17), O2 28 (n= 7), Ar 29 (n=8), O2 29 (n=8). The lungs were removed and stained for analysis of elastic fibers. The Nikkon E600 microscope and the image analysis software (Image-Pro Plus 4.5®) were used for elastic fiber analysis. To compare the analysis between microscope and program was used paired “t” test. Results: There were significant differences between the proportion of elastic fibers performed on the microscope and at the program (p <0.001). Conclusion: The two types of analysis of elastic fibers yield different results. Additional studies comparing the two techniques are required.